Sirtuin

Sirtuin

Sirtuin

Name

Description

Product #

Price

Quantity

Shipping Method

  • View Data Sheet

    Description:

    Sirtuin-6 Human Recombinant

    Mono-ADP-ribosyltransferase sirtuin-6, SIR2-like protein 6, SIRT6, SIR2L6, Sirtuin-6.

    Product # :

    PRO-282

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    SIRT6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 375 amino acids (1-355 a.a.) and having a molecular mass of 41 kDa. Recombinant SIRT6 is fused to 20 amino acids His-tag at N-terminus and purified by conventional chromatography techniques.

    Source

    Escherichia Coli.

    Formulation

    The SIRT6 protein solution (1mg/ml) contains 20mM Tris-HCl pH-8 & 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      SIRT6 is part of the sirtuin family of proteins (Class IV), homologs to the yeast Sir2 protein. SIRT6 is characterized by a sirtuin core domain. Yeast sirtuin proteins are recognized by their ability to regulate epigenetic gene silencing and suppress recombination of rDNA. SIRT6, a chromatin-associated protein is involved in DNA repair. Human Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

    • Synonyms

      Mono-ADP-ribosyltransferase sirtuin-6, SIR2-like protein 6, SIRT6, SIR2L6, Sirtuin-6.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MSVNYAAGLS PYADKGKCGL PEIFDPPEEL ERKVWELARL VWQSSSVVFH TGAGISTASG IPDFRGPHGV WTMEERGLAP KFDTTFESAR PTQTHMALVQ LERVGLLRFL VSQNVDGLHV RSGFPRDKLA ELHGNMFVEE CAKCKTQYVR DTVVGTMGLK ATGRLCTVAK ARGLRACRGE LRDTILDWED SLPDRDLALA DEASRNADLS ITLGTSLQIR PSGNLPLATK RRGGRLVIVN LQPTKHDRHA DLRIHGYVDE VMTRLMEHLG LEIPAWDGPR
      VLERALPPLP RPPTPKLEPK EESPTRINGS IPAGPKQEPC AQHNGSEPAS PKRERPTSPA PHRPPKRVKA KAVPS.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sirt6 Human
  • View Data Sheet

    Description:

    Sirtuin-5 Human Recombinant

    Sirtuin 5, NAD-Dependent Lysine Demalonylase And Desuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5 Mitochondrial, Silent Mating Type Information Regulation 2 (S.Cerevisiae) Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog 5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5.1.-.

    Product # :

    PRO-1774

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    SIRT5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain topological domain containing 300 amino acids (34-310 a.a) and having a molecular mass of 32.5kDa. SIRT5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    SIRT5 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      SIRT5 belongs to the Sirtuin family of proteins, homologs to the yeast Sir2 protein. Sirtuin family members hold a sirtuin core domain and grouped into four classes. The protein encoded by this gene is included in class III of the sirtuin family. Human sirtuins functions were not yet determined; still, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies propose that human sirtuin could operate as intracellular regulatory protein with mono-ADP-ribosyltransferase activity.

    • Synonyms

      Sirtuin 5, NAD-Dependent Lysine Demalonylase And Desuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5 Mitochondrial, Silent Mating Type Information Regulation 2 (S.Cerevisiae) Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog 5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5.1.-.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKW QAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAG TKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQ GPCGTTLPEA LACHENETVS

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sirt5 Human
  • View Data Sheet

    Description:

    Sirtuin 3 Human Recombinant

    Sirtuin 3, SIR2-like protein 3, Sir2-like 3, NAD-dependent deacetylase sirtuin-3, mitochondrial.

    Product # :

    PRO-462

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    SIRT3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (118-399a.a.) and having a molecular mass of 33.5kDa.SIRT3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The SIRT3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 0.1M NaCl, 10% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      SIRT3 is a member of a family of proteins called Sirtuin. Proteins of the Sirtuin family are characterized by a Sirtuin core domain and grouped into four classes. The roles of human Sirtuins are numerous and are important in aging prosseses, stress resistance and metabolic regulation. SIRT3 shows NAD+-dependent deacetylase activity in the mitochondria. Over-expression of SIRT3 causes increased levels of the mitochondrial uncoupling protein 1. Also, in certain breast cancers the levels of SIRT3 protein are found to be extremely high.

    • Synonyms

      Sirtuin 3, SIR2-like protein 3, Sir2-like 3, NAD-dependent deacetylase sirtuin-3, mitochondrial.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MSDKGKLSLQ DVAELIRARA CQRVVVMVGA GISTPSGIPD FRSPGSGLYS NLQQYDLPYP EAIFELPFFF
      HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR
      VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FASLTEAVRS SVPRLLINRD LVGPLAWHPR SRDVAQLGDV
      VHGVESLVEL LGWTEEMRDL VQRETGKLDG PDK.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sirt3 Human
  • View Data Sheet

    Description:

    Sirtuin 2 Human Recombinant

    Sirtuin 2, SIR2L2, SIR2-like protein 2, NAD-dependent deacetylase sirtuin-2, Silent Information Regulator 2.

    Product # :

    PRO-033

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    SIRT2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 372 amino acids (1-352a.a.) and having a molecular mass of 41.7kDa.SIRT2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The SIRT2 protein solution (0.25mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 2mM DTT, 200mM NaCl, 0.5mM EDTA and 30% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      SIRT2 belongs to the sirtuin family of proteins, homologs to the yeast Sir2 protein. Proteins of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and take part in various processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin.

    • Synonyms

      Sirtuin 2, SIR2L2, SIR2-like protein 2, NAD-dependent deacetylase sirtuin-2, Silent Information Regulator 2.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MDFLRNLFSQ TLSLGSQKER LLDELTLEGV ARYMQSERCR RVICLVGAGI STSAGIPDFR SPSTGLYDNL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFMRLLKD KGLLLRCYTQ NIDTLERIAG LEQEDLVEAH GTFYTSHCVS ASCRHEYPLS WMKEKIFSEV TPKCEDCQSL VKPDIVFFGE SLPARFFSCM QSDFLKVDLL LVMGTSLQVQ PFASLISKAP LSTPRLLINK EKAGQSDPFL GMIMGLGGGM DFDSKKAYRD VAWLGECDQG CLALAELLGW KKELEDLVRR EHASIDAQSG AGVPNPSTSA SPKKSPPPAK DEARTTEREK PQ

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sirt2 Human
  • View Data Sheet

    Description:

    Sirtuin-1 Human Recombinant

    Sirtuin 1, SIRT1, SIR2L1, Regulatory Protein SIR2 Homolog 1, hSIR2, hSIRT1, SIR2-Like Protein 1, SIR2alpha, NAD-Dependent Deacetylase Sirtuin-1, NAD-Dependent Protein Deacetylase Sirtuin-1, Sir2-Like 1, Sirtuin Type 1, EC 3.5.1.-.

    Product # :

    PRO-1909

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    SIRT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (254-495) and having a molecular mass of 31.6 kDa.SIRT1 is fused to a 38 amino acid His-tag at N-terminus.

    Source

    Escherichia Coli.

    Formulation

    The SIRT1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Sirtuin-1 (SIRT1) belongs to the sirtuin family of proteins, homologs to the yeast Sir2 protein. Sirtuin family members are characterized by a sirtuin core domain and grouped into 4 classes. The functions of human sirtuins have not yet been established; though, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. It has been proposed that the human sirtuins may serve as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

    • Synonyms

      Sirtuin 1, SIRT1, SIR2L1, Regulatory Protein SIR2 Homolog 1, hSIR2, hSIRT1, SIR2-Like Protein 1, SIR2alpha, NAD-Dependent Deacetylase Sirtuin-1, NAD-Dependent Protein Deacetylase Sirtuin-1, Sir2-Like 1, Sirtuin Type 1, EC 3.5.1.-.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sirt1 Human

About SIRT/ Sirtuin:

Sirtuin proteins (or SIRT) are a class of proteins that have been shown to regulate the aging process, mitochondrial function, gene expression, and cellular metabolism. They carry out these functions by deacetylating specific lysine residues in other proteins.

SIRT Mechanism
Sirtuin proteins work by removing acetyl groups from specific lysine residues in other proteins. In the nucleus, they are found mainly as a homodimer (a protein that consists of two identical polypeptide chains) and bind to specific DNA sequences adjacent to telomeres. They contact histones on the DNA and, in turn, deacetylate the lysine residues on these histones. It results in a change of DNA transcription, regulating the epigenetic state of telomeres.

Sirtuin Interactions
When SIRT are bound to an acetylated histone, they can recruit other proteins which contain a lysine residue. The process of recruitment is facilitated by the structures that form between the protein and DNA. In addition to that, once recruited, these enzymes deacetylated for SIRT's role to continue.
A lot of current research is being done on the SIRT proteins and their role in aging. Deacetylating a particular residue, known as lysine 130 (K130), decreased acetylated HSP90 protein levels. It means an increase in autophagy activity which helps fight off inflammatory responses and prevent age-related diseases.
The findings are very promising as they help understand how to slow down the aging process. However, more research is needed before making any concrete conclusions on whether SIRT proteins play a role in longevity or not.