LBP

LBP

LBP

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  • View Data Sheet

    Description:

    Lipopolysaccaride Mouse Recombinant

    Lipopolysaccharide-binding protein, LBP, Ly88.

    Product # :

    PRO-539

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    Shipped at Room temp

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    • description
    • source
    • formulation
    • biological activity
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    Description

    LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off.

    Source

    Chinese Hamster Ovarian Cells (CHO).

    Formulation

    Recombinant Mouse LBP was lyophilized from a protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.

    Biological Activity

    Up to 2µg/ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (2 x 106/ml).

    More Info

    • Introduction

      Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid.

    • Synonyms

      Lipopolysaccharide-binding protein, LBP, Ly88.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized LBP in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lbp Mouse
  • View Data Sheet

    Description:

    Lipopolysaccarid Binding Protein, Human Recombinant, HEK

    Lipopolysaccharide-binding protein, LBP, MGC22233, Ly88.

    Product # :

    PRO-2581

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    Quantity :

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    • description
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    Description

    LBP Human Recombinant is a single, glycosylated polypeptide chain containing 462 amino acids (26-481a.a) and having a molecular mass of 51.7kDa (calculated). LBP is fused to a 6 a.a His tag at C-terminal.

    Source

    HEK293 Cells.

    Formulation

    LBP filtered (0.4 µm) and lyophilized from 0.5mg/ml solution in PBS, pH7.5 and 5% (w/v) Threalose.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid.

    • Synonyms

      Lipopolysaccharide-binding protein, LBP, MGC22233, Ly88.

    • Physical Appearance

      Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

    • Solubility

      It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.

    • Amino Acid Sequence

      ANPGLVARIT DKGLQYAAQE GLLALQSELL RITLPDFTGD LRIPHVGRGR YEFHSLNIHS CELLHSALRP VPGQGLSLSI SDSSIRVQGR WKVRKSFFKL QGSFDVSVKG ISISVNLLLG SESSGRPTVT ASSCSSDIAD VEVDMSGDLG WLLNLFHNQI ESKFQKVLES RICEMIQKSV SSDLQPYLQT LPVTTEIDSF ADIDYSLVEA PRATAQMLEV MFKGEIFHRN HRSPVTLLAA VMSLPEEHNK MVYFAISDYV FNTASLVYHE EGYLNFSITD DMIPPDSNIR LTTKSFRPFV PRLARLYPNM NLELQGSVPS APLLNFSPGN LSVDPYMEID AFVLLPSSSK EPVFRLSVAT NVSATLTFNT SKITGFLKPG KVKVELKESK VGLFNAELLE ALLNYYILNT FYPKFNDKLA EGFPLPLLKR VQLYDLGLQI HKDFLFLGAN VQYMRVHHHH HH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lbp Protein
  • View Data Sheet

    Description:

    Lipopolysaccaride Human Recombinant

    Lipopolysaccharide-binding protein, LBP, MGC22233.

    Product # :

    PRO-538

    Price :

    Quantity :

    Shipping Method :

    Room Temp Icon

    Shipped at Room temp

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • biological activity
    • More Info

    Description

    The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE.Attention: His-tag has no protease site and can not be split off.

    Source

    Chinese Hamster Ovarian Cells (CHO).

    Formulation

    Recombinant Human LBP was lyophilized from a protein solution (0.3mg/ml) containing phosphate-buffered saline, pH 7.2.

    Biological Activity

    Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).

    More Info

    • Introduction

      Lipopolysaccharides (LPS) are a type of glycolipids on the outer cell wall of Gram-negative bacteria. Lipopolysaccharide binding protein (aka LBP) is a plasma protein which facilitates the diffusion of bacterial LPS (endotoxin). LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with bactericidal permeability-increasing protein (BPI), LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. LBP belongs to a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). The LBP gene is found on chromosome 20, directly downstream of the BPI gene. LBP catalyzes the transfer of LPS monomers from LPS aggregates to HDL particles, to phospholipid bilayers, and to a binding site on soluble CD14 (sCD14). sCD14 is capable of speeding up the transfer by receiving an LPS monomer from an LPS aggregate, and then yielding it to an HDL particle, therefore acting as a soluble "shuttle" for an insoluble lipid.

    • Synonyms

      Lipopolysaccharide-binding protein, LBP, MGC22233.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

    • Solubility

      Every 10µg of recombinant human LBP should be reconstituted using 33µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lbp Human

About LBP:

The liver primarily produces a lipopolysaccharide-binding protein (LBP) as part of the innate immune response to Gram-negative bacterial infection or damage. LBP binds lipopolysaccharide (LPS), a component of Gram-negative bacteria's outer membrane. LPS is highly inflammatory and is found in the blood or other bodily fluids when bacterial cells are damaged. It comprises carbohydrate "O-antigens," core oligosaccharides, and Lipid A, a highly immunogenic and hydrophobic lipid.

LBP Mechanism
This gene's protein is involved in the acute-phase immune response to gram-negative bacterial infections. Lipopolysaccharide (LPS), a glycolipid, is found on the outer cell wall of Gram-negative bacteria. The encoded protein, in collaboration with bactericidal permeability-increasing protein (BPI), binds LPS and interacts with the CD14 receptor, most likely regulating LPS-dependent monocyte responses.Studies on mice have shown that the encoded protein is required for the rapid acute-phase response to LPS but not for LPS clearance from circulation. This protein is a protein family member that includes BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Finally, this gene is located on chromosome 20, just downstream of the BPI gene. Furthermore, lipopolysaccharide-binding protein (LBP) has been studied as a marker of bacterial infections in patients with liver disease. This marker is linked to a higher mortality rate. Furthermore, LBP levels have been studied concerning predicting the outcome of lung cancer and HCV treatment. LBP is a useful tool for clinical and research studies because it is associated with many disease states and complications.