- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
ENZ-1038
Synonyms
Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, TDP1, FLJ11090, MGC104252.
Introduction
TDP1 belongs to the phospholipase D family and contains two PLD phosphodiesterase domains. TDP1 is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. TDP1 may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. Mutations in the TDP1 gene are linked to the disease spinocerebellar ataxia with axonal neuropathy (SCAN1).
Description
TDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 617 amino acids (1-608) and having a molecular mass of 69.5kDa. TDP1 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
TDP1 protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPMSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.