TAR DNA

TAR DNA

TAR DNA

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  • View Data Sheet

    Description:

    TAR DNA Binding Protein Human Recombinant

    ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.

    Product # :

    PRO-1410

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    Description

    TARDBP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (1-260 a.a) and having a molecular mass of 33.6kDa. TARDBP is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    TARDBP protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      TARDBP binds both DNA and RNA and have numerous roles in transcriptional repression, pre-mRNA splicing and translational regulation. TARDBP was initially identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. TARDBP has also been identified in individuals diagnosed with chronic traumatic ncephalopathy, a condition which frequently mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury. TARDBP may also be involved in microRNA biogenesis, apoptosis and cell division.

    • Synonyms

      ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNA.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Tardbp Human
  • View Data Sheet

    Description:

    TAR DNA Binding Protein (1-414 a.a.) Human Recombinant, His Tag

    ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.

    Product # :

    PRO-1459

    Price :

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    Description

    TARDBP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 450 amino acids (1-414 a.a) and having a total molecular mass of 48.8 kDa. TARDBP is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    TARDBP protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M urea.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      TAR DNA Binding Protein (TARDBP) was initially recognized as a transcriptional repressor which binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, TARDBP has been detected in individuals diagnosed with chronic traumatic encephalopathy as well, which is a condition that often imitates ALS andhas been related with athletes who have experienced multiple concussions and other types of head injury.TARDBP has been shown to bind both DNA and RNA, it has multiple functions in transcriptional repression, pre-mRNAsplicing and translational regulation.

    • Synonyms

      ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Tardbp 1 414 Human
  • View Data Sheet

    Description:

    TAR DNA Binding Protein (1-414 a.a.) Human Recombinant

    ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.

    Product # :

    PRO-2383

    Price :

    Quantity :

    Shipping Method :

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    • description
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    Description

    TARDBP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 414 amino acids (1-414 a.a) and having a molecular mass of 44.7kDa.TARDBP is purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    TARDBP protein solution (0.25mg/ml) containing 20mM Tris-HCl (pH8.0), 1mM DTT, 0.1M NaCl, 10% glycerol and 1mM EDTA.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      TAR DNA Binding Protein (TARDBP) was initially recognized as a transcriptional repressor which binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, TARDBP has been detected in individuals diagnosed with chronic traumatic encephalopathy as well, which is a condition that often imitates ALS andhas been related with athletes who have experienced multiple concussions and other types of head injury.TARDBP has been shown to bind both DNA and RNA, it has multiple functions in transcriptional repression, pre-mRNAsplicing and translational regulation.

    • Synonyms

      ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC MRGVRLVEGI LHAPDAGWGN LVYVVNYPKD NKRKMDETDA SSAVKVKRAV QKTSDLIVLG LPWKTTEQDL KEYFSTFGEV LMVQVKKDLK TGHSKGFGFV RFTEYETQVK VMSQRHMIDG RWCDCKLPNS KQSQDEPLRS RKVFVGRCTE DMTEDELREF FSQYGDVMDV FIPKPFRAFA FVTFADDQIA QSLCGEDLII KGISVHISNA EPKHNSNRQL ERSGRFGGNP GGFGNQGGFG NSRGGGAGLG NNQGSNMGGG MNFGAFSINP AMMAAAQAAL QSSWGMMGML ASQQNQSGPS GNNQNQGNMQ REPNQAFGSG NNSYSGSNSG AAIGWGSASN AGSGSGFNGG FGSSMDSKSS GWGM.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Human Tardbp 1 414
  • View Data Sheet

    Description:

    TAR RNA Binding Protein 2 Human Recombinant

    LOQS, TRBP, TRBP1, TRBP2, RISC-loading complex subunit TARBP2, TAR RNA-binding protein 2, Trans-activation-responsive RNA-binding protein, TARBP2.

    Product # :

    PRO-1980

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    Description

    TARBP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-345 a.a) and having a molecular mass of 36.9kDa. TARBP2 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    TARBP2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TAR RNA Binding Protein 2 (TARBP2) is a component of the RISC loading complex which takes part in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. TARBP2, which is also known as the micro-RNA (miRNA) loading complex (miRLC), contains three DRBM (double-stranded RNA-binding) domains. TARBP2 binds to the HIV-1 TAR RNA and stimulates translation of TAR-containing RNAs.

    • Synonyms

      LOQS, TRBP, TRBP1, TRBP2, RISC-loading complex subunit TARBP2, TAR RNA-binding protein 2, Trans-activation-responsive RNA-binding protein, TARBP2.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MLAANPGKTP ISLLQEYGTR IGKTPVYDLL KAEGQAHQPN FTFRVTVGDT SCTGQGPSKK AAKHKAAEVA LKHLKGGSML EPALEDSSSF SPLDSSLPED IPVFTAAAAA TPVPSVVLTR SPPMELQPPV SPQQSECNPV GALQELVVQK GWRLPEYTVT QESGPAHRKE FTMTCRVERF IEIGSGTSKK LAKRNAAAKM LLRVHTVPLD ARDGNEVEPD DDHFSIGVGS RLDGLRNRGP GCTWDSLRNS VGEKILSLRS CSLGSLGALG PACCRVLSEL SEEQAFHVSY LDIEELSLSG LCQCLVELST QPATVCHGSA TTREAARGEA ARRALQYLKI MAGSK.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Tarbp2 Human

About TAR DNA:

TAR DNA, or TAR DNA-binding protein 34 (also known as (TDP-43, transactive response DNA binding protein 43 kDa) is a protein in humans.

TAR DNA Function
TAR DNA is a transcriptional repressor, binding to chromosomal integrated TAR DNA with the purpose of repressing HIV Type 1 (HIV-1) transcription, binding to the trans-active response element DNA sequence of the viral genome. It also regulates alternative splicing of the cystic fibrosis transmembrane conductance regulator, a membrane protein and chloride channel. TAR DNA has multiple functions in transcriptional repression, pre-mRNA splicing, and translation regulation. It is also a key element of the non-homologous end joining enzymatic pathway that repairs DNA double-strand breaks in pluripotent stem cell-derived motor neurons

TAR DNA Mechanism
TAR DNA plays a role in processing molecules called messenger RNA (or mRNA) which are the genetic blueprints that lead to the creation of bproteins. TAR DNA, or the TDP-43 protein, cuts and rearranges mRNA molecules, controlling the production of various versions of different proteins.

TAR DNA Interactions
TAR DNA has a wide range of functions and interactions in multiple steps of RNA metabolism. As such, there are more than 6000 mRNA targets that can be associated with TAR DNA, which includes nearly 30% of the entire transcriptome. Specific RNA targets of TAR DNA include the 3′ UTRs of mRNAs/pre-mRNAs when localized to the cytoplasm.

TAR DNA Structure
TAR DNA is 414 amino acid residues long, consisting of 4 domains. These are an N-terminal domain spanning residues 1-76 with a well-defined fold, 2 folded RNA recognition motifs spanning residues 106-176 and 191-259, and an unstructured C-terminal domain spanning residues 274-414.