PON1 Human (170-232)

Serum paraoxonase, arylesterase 1, EC 3.1.1.2, EC 3.1.8.1, PON 1.

The PON1 Human is created as a recombinant protein with a 4kda N-terminal fusion of His Tag.

The PON1 His-Tagged Fusion Protein, produced in E. coli, is a 13kDa protein containing 63 amino acid residues of the PON1 Human, 170-232 amino acids.

Filtered White lyophilized (freeze-dried) powder.

Each mg was lyophilized with 1xPBS, 0.4% SDS and 4mM DTT.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile!

Please filter the product by an appropriate sterile filter before using it on cell culture.

Store lyophilized PON1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Greater than 90% as determined by SDS-PAGE.

SDS

PON1takes part in the detoxification of organophosphate insecticides such as parathion.

PON1 is related to high-density lipoprotein (HDL) in the plasma and takes an important part in lipid metabolism and antioxidant defense.

PON1 was first known for its ability to protect against oxidative stress and hydrolyze organophosphates.

PON1 reduces oxidative stress by preventing the oxidation of lipids in LDL particles, which is extremely important in mitigating atherosclerosis and other cardiovascular diseases.

PON1 has anti-inflammatory effects which help reduce inflammatory markers in different disease states.

PON1 takes part in cholesterol metabolism, influencing the stability and formation of HDL particles, which are vital for lipid transport and reverse cholesterol efflux.