- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2580
Synonyms
Fibrinogen-like protein 1, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, HFREP-1, Liver fibrinogen-related protein 1, LFIRE-1, FGL1, HFREP1.
Introduction
FGL1 belongs to the fibrinogen family. Fibrinogen Like 1 is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which comprises the 4 conserved cysteines of fibrinogens and fibrinogen related proteins. FGL1 lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. Fibrinogen takes part in the development of hepatocellular carcinomas.
Description
FGL1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 23-312) containing 300 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 35.3kDa (calculated).
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
FGL1 filtered (0.4 µm) and lyophilized from 0.5mg/ml in in 100 mM carbonate buffer, pH 9.2.
Solubility
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. FGL1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MKHHHHHHAS LEDCAQEQMR LRAQVRLLET RVKQQQVKIK QLLQENEVQF LDKGDENTVI DLGSKRQYAD CSEIFNDGYK LSGFYKIKPL QSPAEFSVYC DMSDGGGWTV IQRRSDGSEN FNRGWKDYEN GFGNFVQKHG EYWLGNKNLH FLTTQEDYTL KIDLADFEKN SRYAQYKNFK VGDEKNFYEL NIGEYSGTAG DSLAGNFHPE VQWWASHQRM KFSTWDRDHD NYEGNCAEED QSGWWFNRCH SANLNGVYYS GPYTAKTDNG IVWYTWHGWW YSLKSVVMKI RPNDFIPNVI.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.