KRT8 Human (421-484)

Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.

The KRT8 Human is created as a recombinant protein with a 4kda N-terminal fusion of His Tag. The KRT8 His-Tagged Fusion Protein, produced in E. coli, is a 10kDa protein containing 64 amino acid residues of the KRT8 Human, 421-484 amino acids.

Filtered White lyophilized (freeze-dried) powder.

Each mg was lyophilized with 1xPBS, 0.4% SDS and 4mM DTT.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Store lyophilized KRT8 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

GGLSSAYGGL TSPGLSYSLG SSFGSGAGSS SFSRTSSSRA VVVKKIETRD GKLVSESSDV LPK

Greater than 90% as determined by SDS-PAGE.

SDS

Cytokeratin 8, also known as KRT8, is a part of the type II keratin family clustered on the long arm of chromosome 12 which plays an important role in the structural integrity of epithelial cells. KRT8 pairs with keratin 18 (a type I keratin) to form heterodimers, which assemble into intermediate filaments. KRT8 takes part in retaining cellular structural integrity and also participates in signal transduction and cellular differentiation.