- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2555
Synonyms
Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5, Microsomal cytochrome b5 type A.
Introduction
Cytochrome b5 isoform 1 or CYB5A, is part of the cytochrome b5 family which is bound to the cell membrane. CYB5A has a key role in the stearyl-CoA-desaturase activity as it reduces ferric hemoglobin to ferrous hemoglobin. Cytochrome b5 isoform 1 performs as an electron carrier for numerous oxygenases that are imbedded in the cell membrane.
Description
CYB5A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 117 amino acids (1-108a.a.) and having a molecular mass of 13.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
CYB5A is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CYB5A protein solution (1mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPMAEQSDE AVKYYTLEEI QKHNHSKSTW LILHHKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDAREMSK TFIIGELHPDDRPKLNKPPE TLITTIDSSS SHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.