{"title":"Ubiquitin Conjugating Enzyme","description":"\u003ch3 class=\"highlited\"\u003e\u003cspan\u003eAbout Ubiquitin Conjugating Enzyme:\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eUbiquitin-conjugating enzymes catalyze the second step of the ubiquitination reaction that enables the degradation of proteins via the proteasome.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUbiquitin Conjugating Enzyme Mechanism\u003c\/strong\u003e\u003cbr\u003eThe purpose of the ubiquitin-conjugating enzyme is to help the cell recycle short peptide fragments.\u003cbr\u003eThe first step is to attach ubiquitin - a relatively short protein made of 76 amino acids - to the lysine on the target protein using a covalent reaction. Once the initial ubiquitin molecule is in place, and the protein has been tagged, it is then possible for the polyubiquitin chain to form. The proteasome’s 19S regulatory particle recognizes the chain. The proteasome recognizes the chain, triggering the unfolding of the target protein via ATP and allowing passage to the proteasome’s 20S core particle. Proteases then degrade the target into short peptide chains that the cell can use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUbiquitin Conjugating Enzyme Function\u003c\/strong\u003e\u003cbr\u003eThere are three classes of ubiquitin-related enzymes: E1, E2, and E3. All of these classes have different properties and operate in distinct ways in the body.\u003cbr\u003eE1 is the least physiologically-regulated of all of the enzymes in this class. It activates ubiquitin using the ATP-dependent mechanism described in the previous section. The ATP-activated ubiquitins then bind to the protein substrate and degrade them using the ubiquitin-proteasome pathway. The process is not dependent on the substrate.\u003cbr\u003eE2 ubiquitin-conjugating enzymes are believed to be more selective in their choice of target. Researchers initially thought that the purpose of E2 enzymes was simply to transfer ubiquitin to E3s. However, they may play a more active role. Current evidence suggests that E2s may be able to directly conjugate ubiquitin into substrates.\u003cbr\u003eUnfortunately, a definitive answer to this question is likely a long way from being answers. E2s are structurally diverse and found in different forms across a range of species. In yeast, for instance, 13 genes encode for E2s. In mammals, there appears to be in the order of 25 to 30.\u003cbr\u003eE3s are widely believed to be even more diverse than E2s. In combination, there is evidence that they may have a high degree of site-specificity, allowing them to work on a range of substrates without damaging surrounding tissue.\u003cbr\u003eE3s are so diverse that they may use catalytic mechanisms not described above. Other possible chemical mechanisms could break proteins down into their peptide constituents.\u003cbr\u003eUbiquitin-conjugating enzymes should not be confused with ubiquitin-activating enzymes. Ubiquitin-activating enzymes are those described above that are not substrate-specific. Their role is to active the ubiquitin by covalently attaching the molecule to the protein site cysteine residue. Once that has occurred, it is then time for the E2 and E3 conjugating enzymes to take over the process. After conjugation, the E2 molecule binds to several of the ubiquitin ligases or the E3 via the unique binding region.\u003cbr\u003eThe job of the E3 ubiquitin-conjugating enzyme is to bind to the target protein substrate and then transfer the ubiquitin from the cysteine residue to a new lysine residue on the surface of the target protein.\u003c\/p\u003e","products":[{"product_id":"sae1-human","title":"SAE1 Human","description":"\u003cp\u003eSUMO1 Activating Enzyme Subunit 1 Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eAOS1, HSPC140, SUA1, UBLE1A, SAE1, SUMO1 Activating Enzyme Subunit 1, 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hUBA3.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"5µg","offer_id":43174981828692,"sku":"ENZ-576","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20µg","offer_id":43174981861460,"sku":"ENZ-576","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43174981894228,"sku":"ENZ-576","price":2700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-uba3-human-ENZ-576.png?v=1776713739"},{"product_id":"uba5-human","title":"UBA5 Human","description":"\u003cp\u003eUbiquitin-Like Modifier Activating Enzyme 5 Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eUbiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBA5, UBE1DC1.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"2µg","offer_id":43174982680660,"sku":"ENZ-602","price":60.0,"currency_code":"USD","in_stock":true},{"title":"10µg","offer_id":43174982713428,"sku":"ENZ-602","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43174982746196,"sku":"ENZ-602","price":5200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-uba5-human-ENZ-602.png?v=1776713745"},{"product_id":"ube2a-human","title":"UBE2A Human","description":"\u003cp\u003eUbiquitin Conjugating Enzyme E2A Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eHHR6A, HR6A, RAD6A, UBC2, UBE2A, EC=6.3.2.19, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-protein ligase A, Ubiquitin carrier protein A, RAD6 homolog 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UBE2B.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"2µg","offer_id":43174985334868,"sku":"ENZ-340","price":60.0,"currency_code":"USD","in_stock":true},{"title":"10µg","offer_id":43174985367636,"sku":"ENZ-340","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43174985400404,"sku":"ENZ-340","price":5200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2b-human-ENZ-340.png?v=1776713760"},{"product_id":"ube2c-human","title":"UBE2C Human","description":"\u003cp\u003eUbiquitin Conjugating enzyme E2C Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eUbiquitin-conjugating enzyme E2 C, EC 6.3.2.19, Ubiquitin-protein ligase C, Ubiquitin carrier protein C, Ubc10, UBCH10, dJ447F3.2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"5µg","offer_id":43174986121300,"sku":"ENZ-346","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20µg","offer_id":43174986154068,"sku":"ENZ-346","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43174986186836,"sku":"ENZ-346","price":2700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2c-human-ENZ-346.png?v=1776713767"},{"product_id":"ube2d1-human","title":"UBE2D1 Human","description":"\u003cp\u003eUbiquitin Conjugating Enzyme E2D1 Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eUbiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, SFT, UBC4\/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1, UBE2D1, SFT, UBC5A, 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UbcM4.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"10µg","offer_id":43175002177620,"sku":"ENZ-342","price":60.0,"currency_code":"USD","in_stock":true},{"title":"50µg","offer_id":43175002210388,"sku":"ENZ-342","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175002243156,"sku":"ENZ-342","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2l3-human-ENZ-342.png?v=1776713841"},{"product_id":"ube2l3-human-his","title":"UBE2L3 Human His","description":"\u003cp\u003eUbiquitin-Conjugating Enzyme E2L 3 Human Recombinant, His Tag\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eUbiquitin-conjugating enzyme E2 L3, EC 6.3.2.19, Ubiquitin-protein ligase L3,Ubiquitin carrier protein L3, UbcH7, E2-F1, L-UBC, 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MGC131857.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"5µg","offer_id":43175007060052,"sku":"ENZ-104","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20µg","offer_id":43175007092820,"sku":"ENZ-104","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175007125588,"sku":"ENZ-104","price":2700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2n-human-ENZ-104.png?v=1776713861"},{"product_id":"ube2q2-human","title":"UBE2Q2 Human","description":"\u003cp\u003eUbiquitin Conjugating Enzyme E2Q2 Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eUbiquitin-conjugating enzyme E2 Q2, UBE2Q2, E2 ubiquitin-conjugating enzyme Q2, Ubiquitin carrier protein Q2, Ubiquitin-protein ligase Q2, Ubiquitin Conjugating Enzyme E2Q2, Ubiquitin-conjugating enzyme E2 Q2 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6.3.2.19.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"2µg","offer_id":43175008763988,"sku":"ENZ-511","price":60.0,"currency_code":"USD","in_stock":true},{"title":"10µg","offer_id":43175008796756,"sku":"ENZ-511","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175008829524,"sku":"ENZ-511","price":5200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2r2-human-ENZ-511.png?v=1776713870"},{"product_id":"ube2s-human","title":"UBE2S Human","description":"\u003cp\u003eUbiquitin Conjugating Enzyme E2S Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eUbiquitin-conjugating enzyme E2 S, Ubiquitin-protein ligase S, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, E2-EPF5, E2-EPF, UBE2S, E2EPF, EPF5.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"10µg","offer_id":43175010598996,"sku":"ENZ-444","price":60.0,"currency_code":"USD","in_stock":true},{"title":"50µg","offer_id":43175010631764,"sku":"ENZ-444","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175010664532,"sku":"ENZ-444","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2s-human-ENZ-444.png?v=1776713878"},{"product_id":"ube2t-human","title":"UBE2T Human","description":"\u003cp\u003eUbiquitin-Conjugating Enzyme E2T Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eEC 6.3.2.19, HSPC150, PIG50, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, Cell proliferation-inducing gene 50 protein, UBE2T.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"5µg","offer_id":43175011549268,"sku":"ENZ-506","price":60.0,"currency_code":"USD","in_stock":true},{"title":"25µg","offer_id":43175011582036,"sku":"ENZ-506","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175011614804,"sku":"ENZ-506","price":2250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2t-human-ENZ-506.png?v=1776713882"},{"product_id":"ube2v2-human","title":"UBE2V2 Human","description":"\u003cp\u003eUbiquitin-Conjugating Enzyme E2 Variant 2 Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eDDVit-1, DDVIT1, EDAF-1, EDPF-1, EDPF1, MMS2, UEV-2, UEV2, Ubiquitin-conjugating enzyme E2 variant 2, Enterocyte differentiation-associated factor 1, Enterocyte differentiation-promoting factor 1, Vitamin D3-inducible protein, UBE2V2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"5µg","offer_id":43175012401236,"sku":"ENZ-550","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20µg","offer_id":43175012434004,"sku":"ENZ-550","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175012466772,"sku":"ENZ-550","price":2700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2v2-human-ENZ-550.png?v=1776713886"},{"product_id":"ube2w-human","title":"UBE2W Human","description":"\u003cp\u003eUbiquitin Conjugating Enzyme E2W Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eProbable ubiquitin-conjugating enzyme E2 W, Ubiquitin carrier protein W, Ubiquitin-protein ligase W, UBE2W, hUBC-16, FLJ11011.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"prospecbio","offers":[{"title":"5µg","offer_id":43175013449812,"sku":"ENZ-117","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20µg","offer_id":43175013482580,"sku":"ENZ-117","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":43175013515348,"sku":"ENZ-117","price":2700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0559\/3219\/0804\/files\/Prospecbio-ube2w-human-ENZ-117.png?v=1776713890"},{"product_id":"ube2z-human","title":"UBE2Z Human","description":"\u003cp\u003eUbiquitin Conjugating Enzyme E2Z Human Recombinant\u003c\/p\u003e\n\n\u003cdiv class=\"synonyms_hide\"\u003e\n  \u003cp\u003eHOYS7, USE1, Ubiquitin-conjugating enzyme E2 Z, E2 ubiquitin-conjugating enzyme Z, Uba6-specific E2 conjugating enzyme 1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z, 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