Chymotrypsin Porcine

a-chymotrypsin, alpha chymotrypsin.

Chymotrypsin purified from porcine pancreas, CAS: 9004-07-3, EC: 3.4.21.1 having a molecular mass of ~25kDa.

Porcine Pancreas.

Sterile Filtered White lyophilized (freeze-dried) powder.

The protein (1mg/ml) was lyophilized with no additives.

It is recommended to reconstitute the lyophilized Chymotrypsin in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Chymotrypsin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Chymotrypsin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Greater than 1500 USP U/mg.

SDS

Porcine chymotrypsin, derived from the pancreas of pigs, stands as a cornerstone in enzymology, serving as a paradigmatic model for understanding proteolytic mechanisms and substrate specificity. Renowned for its catalytic prowess and structural intricacies, porcine chymotrypsin has garnered significant attention from researchers across various scientific disciplines.

The fascination with porcine chymotrypsin stems from its ability to cleave peptide bonds selectively after large hydrophobic amino acids, such as tryptophan, tyrosine, and phenylalanine.