- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-207
Synonyms
Brain-Derived Neurotrophic Factor, BDNF, MGC34632.
Introduction
BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Description
Brain-derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids (and an N-terminal Met) and having a total molecular mass of 27kDa.
BDNF Human Recombinant is purified by proprietary chromatographic techniques.
BDNF Human Recombinant is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized without any additives.
Solubility
It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
BDNF is greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.
Biological Activity
The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2µg/ml.
Protein content
BDNF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.
References
Title:Generation of Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant†‡
Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press.
Link:BDNF prospec publication
Safety Data Sheet
Background
Understanding What Brain-Derived Neurotrophic Factor (BDNF) Human Recombinant Is
A naturally occurring protein could boost your brain power, enhance your memory, and help you feel better. It's known as Brain-Derived Neurotrophic Factor (BDNF), which plays a key role in the overall cognitive function.
BDNF enables brain cells to grow and connect, helping neuronal populations in or connected to the central nervous system survive. As biotechnology advances, the production of this protein in laboratories has become a reality. However, there are still many things to learn about this.
Are you curious about this topic? Find more information about BDNF human recombinant below!
What Is Brain-Derived Neurotrophic Factor (BDNF)?
As mentioned, BDNF is a protein that the body produces naturally. Serving as a neurotransmitter modulator, it's responsible for the development, maintenance, and survival of neurons (nerve cells) in the brain.
This protein is also related to neuronal plasticity, so it may affect people's learning skills and memory.
How Does BDNF Work?
BDNF binds specific receptors on neurons, triggering events that promote neuronal growth, proliferation, and survival. This process helps nerve cells form new connections, known as synapses.
Furthermore, BDNF promotes processes that repair injured neurons and protect existing ones from damage.
Why Is BDNF Necessary?
Since BDNF is essential for cognitive functions, low levels of this protein can cause various issues. Actually, this is often associated with neuronal loss and different neurodegenerative diseases, including the following:
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Alzheimer's disease
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Parkinson's disease
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Multiple sclerosis
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Huntington's disease
In this regard, BDNF can be used to manage or prevent multiple diseases or related conditions, such as diabetes mellitus, depression, and anxiety.
What Is BDNF Human Recombinant?
As explained above, experts have started producing a laboratory-made version of the BDNF protein found in humans. Using recombinant DNA technology, the naturally occurring BDNF gene is inserted into E. Coli bacteria.
After this step, E. Coli bacteria will function as a factory and produce this protein in large quantities.
Benefits of BDNF Human Recombinant
The use of BDNF human recombinant has been linked to several benefits. Most impact people's cognitive function and brain health. Below are some of them:
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Improve learning skills
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Enhance memory performance
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Shield brain cells from damage
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Maintain neurons' health and function
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Prevent neurodegenerative diseases
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Reduce anxiety
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Regulate mood
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Enhance cognitive function
Background
Final Thoughts
Although more research is needed on the safety and effectiveness of BDNF human recombinant, trials suggest that this laboratory-produced protein may be effective in managing and treating several neurological and psychiatric disorders. It's important for experts to stay up to date on the latest developments and research to learn more about potential risks and benefits.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.