ANGPT2 Human

Angiopoietin-2, Angiopoietin2, ANGPT2, ANG2, ANG-2, ANGPT-2.

ANGPT2 aka Angiopoietin-2 competes for binding to the TIE2 receptor and blocks angiopoietin-1 aka ANGPT1 induced TIE2 autophosphorylation during vasculogenesis. ANGPT-2 is a naturally occurring antagonist of ANGPT-1. ANGPT2 induces tyrosine phosphorylation of TEK/TIE2 in the lack of Angiopoietin-2. In the deficiency of VEGF an angiogenic inducer, ANGPT-2 induces endothelial cell apoptosis resulting in vascular regression. ANGPT2 along with VEGF enable endothelial cell migration and proliferation, resulting in permissive angiogenic signal.

ANGPT2 produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain (19-496 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 484 amino acids and having a molecular mass of 55.7kDa.
ANGPT2 shows multiple bands between 50-100kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Chinese Hamster Ovary Cells (CHO).

Sterile filtered colorless solution.

ANGPT2 protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Greater than 90.0% as determined by SDS-PAGE.

SDS

YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQ
RLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQ
TRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKN
SFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQ
HDLMETVNNL LTMMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEE
IKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQR
YVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDG
DNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT MMIRPADFHH HHHH

Angiopoietin 2 Human Recombinant: Unraveling its Role in Angiogenesis and Implications for Therapeutic Interventions

Abstract

This research paper provides a comprehensive analysis of Angiopoietin 2 Human Recombinant (Ang-2), a critical protein involved in angiogenesis, the process of new blood vessel formation. By investigating its structural characteristics, intricate signaling mechanisms, diverse biological functions, and its implications in disease pathology, we shed light on the potential therapeutic applications of Ang-2 in angiogenesis-related disorders.

1. Introduction

Angiogenesis, the formation of new blood vessels from existing ones, is a complex and highly regulated process essential for various physiological and pathological conditions. Angiopoietin 2 (Ang-2), a member of the angiopoietin family, has emerged as a key modulator of angiogenesis, exerting context-dependent effects on endothelial cells and blood vessel remodeling. Understanding the molecular intricacies of Ang-2 is crucial for developing targeted therapeutic strategies for angiogenesis-related disorders.

2. Structural Characteristics of Ang-2

Ang-2 is a secreted glycoprotein composed of an N-terminal coiled-coil domain, a linker region, and a C-terminal fibrinogen-like domain. The unique structural features of Ang-2 enable its interaction with the endothelial-specific receptor Tie-2, initiating a complex signaling cascade that regulates angiogenic responses.

3. Signaling Mechanisms of Ang-2

Ang-2 functions as both a Tie-2 agonist and antagonist, depending on the local microenvironment and the presence of other angiogenic factors. Binding of Ang-2 to Tie-2 can lead to diverse downstream effects, including modulation of endothelial cell survival, proliferation, migration, and vascular permeability. The interplay between Ang-2 and other angiogenic factors tightly regulates angiogenesis.

4. Biological Functions of Ang-2

Ang-2 plays a multifaceted role in angiogenesis by influencing endothelial cell behavior and vascular remodeling. It promotes vessel destabilization and sprouting, facilitating endothelial cell migration and new vessel formation. Additionally, Ang-2 modulates vascular permeability and interacts with other signaling pathways involved in angiogenic processes.

5. Implications of Ang-2 in Disease Pathology

Dysregulation of Ang-2 expression and signaling has been associated with numerous pathological conditions, including cancer, chronic inflammation, and cardiovascular diseases. Elevated Ang-2 levels are often observed in these disorders, contributing to abnormal blood vessel growth, tumor progression, and tissue damage.

6. Therapeutic Implications of Ang-2

Given its critical role in angiogenesis and its involvement in various disease states, Ang-2 represents a promising target for therapeutic interventions. Strategies aimed at modulating Ang-2 signaling, such as the development of neutralizing antibodies or small molecule inhibitors, hold great potential for managing angiogenesis-related disorders and improving patient outcomes.

7. Conclusion and Future Perspectives

Our understanding of Ang-2 and its multifaceted involvement in angiogenesis has advanced significantly. However, further research is required to elucidate the complex interplay between Ang-2 and other angiogenic factors, as well as to explore the potential of Ang-2-targeted therapies. Continued investigations will pave the way for the development of innovative treatment approaches for angiogenesis-related disorders, ultimately improving patient care.