- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PKA-107
Synonyms
Adenylate kinase 2 mitochondrial isoform a, Ak-2, D4Ertd220e, mitochondrial, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferas, Adenylate monophosphate kinase.
Introduction
Adenylate kinases play a role in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. There are 3 types of adenylate kinase isozymes, AK1, AK2, and AK3 in vertebrates. Expression of these isozymes are tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and is involved in apoptosis. AK2 is mutated in individuals with reticular dysgenesis.
Description
AK2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-239 a.a) and having a molecular mass of 29kDa.
AK2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
AK2 protein solution (0.5mg/ml) containing 20mM Tris-Hcl buffer (pH8.5), 10% glycerol and 1mM DTT.
Stability
AK2 Mouse Recombinant although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAPNVL ASEPEIPKGI RAVLLGPPGA GKGTQAPKLA ENFCVCHLAT GDMLRAMVAS GSEL TMDAGKLVSD EMVVELIEKN LETPSCKNGF LLDGFPRTVR QAEMLDDLME KRKEKLDSVI EFSIQDSLLI RRITGRLIHP KSGRS
NPPKEPMKDD ITGEPLIRRS DDNEKALKTR LEAYHTQTTP LVEYYRKRGI HCAIDASQTP DIVFASILAA FSKATCKDLV MFI
Biological Activity
Specific activity is > 40 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.