prospec
FGF 2 Human

FGF 2 Human

  • Name
  • Description
  • Cat#
  • Pricings
  • Quantity
  • FGF 2 Human

  • Fibroblast Growth Factor-Basic Human Recombinant
  • CYT-218
  • Shipped at Room temp.

Catalogue number

CYT-218

Synonyms

Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.

Introduction

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The HPR -binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.2kDa.
The FGF-b is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM Tris-HCl, pH7.4 and 1M NaCl.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by Analysis by SDS-PAGE.

Amino acid sequence

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of murine balb/c 3T3 cells is <0.1ng/ml, corresponding to a specific activity of graeter than 1.0x107 Units/mg.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

References

1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas
Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010
Link:FGF 2 prospec publication

2.Title:Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation
Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013.
Link:FGF 2 Human prospec publication

3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain .
Publication:

  • Cell Transplantation
  • ISSN: 0963-6897
  • DOI: 10.3727/096368910X498944
  • Volume 19, Issue 8, pages 985-998
  • Copyright © 2010 Cognizant Comm. Corp
    Link:Fibroblast Growth Factor-Basic prospec publication

    4.Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues.
    Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press.
    Link:FGF-basic prospec publication

    5.Title: Successful implantation of physiologically functional bioengineered mouse internal anal sphincter
    Publication:  American Journal of Physiology-Gastrointestinal and Liver Physiology 299.2 (2010): G430-G439.
    Link: FGF-b prospec publication

Background

FGF 2 HUMAN: Insights into Fibroblast Growth Factor-2

 

Basic Fibroblast Growth Factor or FGF 2 HUMAN is a protein with crucial roles in cell growth, tissue repair, and embryonic development. This is part of the larger fibroblast growth factor family and is vital for various biological processes, including the modulation of cell survival activities.

 

Production and Properties

 

Produced in E. coli, FGF 2 is a non-glycosylated polypeptide chain possessing 154 amino acids with a molecular weight of about 17.2 kDa. It is purified through advanced chromatographic techniques, ensuring high purity and activity for laboratory use.

 

Physical Characteristics and Preparation

 

The physical form of FGF 2 HUMAN is a sterile, white lyophilized powder. For experimental use, it is reconstituted with sterile water to at least 100µg/ml. This reconstitution is crucial for maintaining the integrity and effectiveness of the protein in various research applications.

 

Storage and Handling

 

To maintain stability, lyophilized FGF 2 should be stored at -18°C and used within three weeks if kept at room temperature. Once reconstituted, it should be kept at 4°C and used within 2-7 days or stored at -18°C for longer-term storage.

 

Proper handling and avoiding repeated freeze-thaw cycles are essential to preserve the protein's functionality.

 

Purity and Biological Activity

 

FGF 2 is characterized by a purity greater than 98%, verified by SDS-PAGE analysis. Its biological activity is primarily defined by its efficacy in promoting the proliferation of specific cell lines, with an effective dose (ED50) typically below 0.1 ng/ml.

 

Research Applications and Impact

 

In the research context, FGF 2 is used extensively to study its effects on cell migration, proliferation, and angiogenesis. Moreover, its role in disease models, particularly in cancer and tissue repair studies, makes it a valuable resource for developing new therapeutic approaches.

 

Usage Guidelines

 

FGF 2 HUMAN is strictly for laboratory research use and is not suitable for drug development, food production, or cosmetic applications. Researchers are advised to comply with safety and handling guidelines to ensure that experiments are conducted under optimal conditions.

 

The Broad Impact on Development and Disease

 

FGF-2 is known for its multifunctional role across numerous biological processes such as tissue repair, embryonic development, angiogenesis, and even tumorigenesis.

 

This growth factor, existing in various synonymous forms such as Basic FGF, FGF-b, and HBGF-2, is essential in cellular processes that underpin both health and disease.

 

Furthermore, FGF-2's ability to bind to cellular receptors triggers a cascade of signaling pathways, including PI3K/Akt, MAPK/ERK, and PLCγ, which in turn influence cell growth, migration, and survival.

 

These pathways are pivotal in mediating the factor's diverse effects on cell behavior, contributing to its critical roles in wound healing, angiogenesis, and tissue remodeling.




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