- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
IHA-010
Introduction
Influenza A virus subtype H5N1 is a subtype of the Influenza A virus. Subtype H5N1 is commonly known as avian influenza or bird flu. H5N1 may cause more than one influenza pandemicas it is expected to continue mutating in birds. The dominant strain of HPAI A (H5N1) evolved creating the Z genotype. It has also been called Asian lineage HPAI/A/H5N1 which is divided into 2 antigenic clades. "Clade 1 includes human and bird isolates from Vietnam, Thailand, and Cambodia and bird isolates from Laosand Malaysia. Clade 2 viruses were first identified in bird isolates from China, Indonesia, Japan, and South Korea before spreading westward to the Middle East, Europe, and Africa.
Description
Recombinant Full-Length H5N1 A/Vietnam/1203/04 is glycosylated with N-linked sugars, produced using baculovirus and when processed under denaturing conditions splits into the HA1 and HA2 domains which are approximately 45,000 and 25,000 Daltons respectively H5N1 A/Vietnam/1203/04 accession number is ISDN38687.
Source
Baculovirus Insect Cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The Recombinant H5N1 A/Vietnam/1203/04 solution contains 10mM Sodium Phosphate, pH 7.1, 0.005% Tween-20 and 150mM NaCl.
Stability
H5N1 A/Vietnam/1203/04 Recombinant should be stored at 4°C. Do not freeze!
Immunological Activity
Western-Blot 0.1µg -1µg per strip, ELISA 1µg/Well.
Purity
Greater than 90.0% as determined byAnalysis by SDS-PAGE, HA1 and HA2 bands are observed using SDS-PAGE under reducing conditions.
Amino acid sequence
V K M E K I V L L F A I V S L V K S D Q I C I G Y H A N N S T E Q V D T I M E
K N V T V T H A Q D I L E K K H N G K L C D L D G V K P L I L R D C S V A G
W L L G N P M C D E F I N V P E W S Y I V E K A N P V N D L C Y P G D F N D
Y E E L K H L L S R I N H F E K I Q I I P K S S W S S H E A S L G V S S A C P
Y Q G K S S F F R N V V W L I K K N S T Y P T I K R S Y N N T N Q E D L L V
L W G I H H P N D A A E Q T K L Y Q N P T T Y I S V G T S T L N Q R L V P R I
A T R S K V N G Q S G R M E F F W T I L K P N D A I N F E S N G N F I A P E
Y A Y K I V K K G D S T I M K S E L E Y G N C N T K C Q T P M G A I N S S M
P F H N I H P L T I G E C P K Y V K S N R L V L A T G L R N S P Q R E R R R
K K R G L F G A I A G F I E G G W Q G M V D G W Y G Y H H S N E Q G S G
Y A A D K E S T Q K A I D G V T N K V N S I I D K M N T Q F E A V G R E F N
N L E R R I E N L N K K M E D G F L D V W T Y N A E L L V L M E N E R T L D
F H D S N V K N L Y D K V R L Q L R D N A K E L G N G C F E F Y H K C D N
E C M E S V R N G T Y D Y P Q Y S E E A R L K R E E I S G V K L E S I G I Y
Q I L S I Y S T V A S S L A L A I M V A G L S L W M C S N G S L Q C R I C I.
K N V T V T H A Q D I L E K K H N G K L C D L D G V K P L I L R D C S V A G
W L L G N P M C D E F I N V P E W S Y I V E K A N P V N D L C Y P G D F N D
Y E E L K H L L S R I N H F E K I Q I I P K S S W S S H E A S L G V S S A C P
Y Q G K S S F F R N V V W L I K K N S T Y P T I K R S Y N N T N Q E D L L V
L W G I H H P N D A A E Q T K L Y Q N P T T Y I S V G T S T L N Q R L V P R I
A T R S K V N G Q S G R M E F F W T I L K P N D A I N F E S N G N F I A P E
Y A Y K I V K K G D S T I M K S E L E Y G N C N T K C Q T P M G A I N S S M
P F H N I H P L T I G E C P K Y V K S N R L V L A T G L R N S P Q R E R R R
K K R G L F G A I A G F I E G G W Q G M V D G W Y G Y H H S N E Q G S G
Y A A D K E S T Q K A I D G V T N K V N S I I D K M N T Q F E A V G R E F N
N L E R R I E N L N K K M E D G F L D V W T Y N A E L L V L M E N E R T L D
F H D S N V K N L Y D K V R L Q L R D N A K E L G N G C F E F Y H K C D N
E C M E S V R N G T Y D Y P Q Y S E E A R L K R E E I S G V K L E S I G I Y
Q I L S I Y S T V A S S L A L A I M V A G L S L W M C S N G S L Q C R I C I.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.